sterilization / autoclave
STERILIATION / AUTOCLAVE Sterilization : is the process used to make the item free from any viable organism. Different type of sterilization process: · Moist heat sterilization (steam sterilization): Mechanism of action (M/A) – Irreversible denaturation and coagulation of enzymes and structural protein. Items to be sterilized by this method – Thermostable aqueous solutions, glassware, plastic wares, garments and rubber articles. · Dry heat sterilization : M/A – Oxidation of cell constituents Uses – glass, metal objects thermostable powders and fatty substances. · Other heating sterilization methods: Flaming = Expose the item to flame, Incineration = Burn the item to ash, Tyndalization – In this method items are boiled for certain time (̴ 20min ) then cool and incubate at room temperature, This step is repeated...